Lanes: M1, step 1 kb DNA steps; HT, genomic DNA of HT1080; step three, 11, 18*, 19 and you can 21, genomic DNA out of focused clones; pP4X (10 7 , ten 8 , ten nine ), duplicates out of linearized pattP4X-PGKssPuro piled because the self-confident manage
Emphasizing endogenous attH4X in the HT1080 muscle. (A) Diagram exhibiting the fresh new 21 nucleotide sequences spanning the key binding and you can spacer sequences of various att internet featuring of the Line-step 1 retrotransposon.